Recombinant humaan Ferredoxin 1/FDX1-eiwit

Ferredoxin1 is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450. This particular oxidation/reduction system is found in steroidogenic tissues, and is involved with the synthesis of bile acid and vitamin D. It participates in the synthesis of thyroid hormones and transfers electrons from adrenodoxin reductase to the cholesterol side chain cleavage cytochrome P450. Recombinant human Ferredoxin 1 protein, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Adrenodoxin, Adrenal ferredoxin, Hepatoredoxin, ADX, FDX, LOH11CR1D, Adrenodoxin, mitochondrial, FDX1, Ferredoxin 1

E.coli

Human

T7-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

15 kDa (139aa) confirmed by MALDI-TOF

Ferredoxin1, Adrenodoxin, Adrenal ferredoxin, Hepatoredoxin, ADX, FDX, LOH11CR1D, Adrenodoxin, mitochondrial, FDX1, Ferredoxin 1, ATGP0359-10 µg, ATGP0359-20 µg, ATGP0359-50 µg, ATGP0359-100 µg, ATGP0359-250 µg, ATGP0359-500 µg, ATGP0359-1 mg, ATGP0359-10, ATGP0359-20, ATGP0359-50, ATGP0359-100, ATGP0359-250, ATGP0359-500, ATGP0359-1

Mandai T., et al. (2009) . FEBS J. 276 (8) :2416-29. ; ; Pichiorri F., et al. (2009) . J Biol Chem. 284 (2) :1040-9.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Redox Proteins

NP_004100.1

P10109

Human

SSSED KITVHFINRD GETLTTKGKV GDSLLDVVVE NNLDIDGFGA CEGTLACSTC HLIFEDHIYE KLDAITDEEN DMLDLAYGLT DRSRLGCQIC LTKSMDNMTV RVPETVADAR QSIDVGKTS