Recombinant humaan fetuïne A/AHSG-eiwit

AHSG, also known as fetuin-A, belongs to the fetuin class of plasma binding proteins. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. It is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has been postulated that it participates in the development of the tissues. It forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate. Recombinant human AHSG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alpha-2-HS-glycoprotein, Alpha-2-HS-glycoprotein, A2HS, AHS, FETuA, HSGA

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

39.7 kDa (372aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Fetuin A, AHSG, Alpha-2-HS-glycoprotein, Alpha-2-HS-glycoprotein, A2HS, AHS, FETuA, HSGA, ATGP2080-10 µg, ATGP2080-20 µg, ATGP2080-50 µg, ATGP2080-100 µg, ATGP2080-250 µg, ATGP2080-500 µg, ATGP2080-1 mg, ATGP2080-10, ATGP2080-20, ATGP2080-50, ATGP2080-100, ATGP2080-250, ATGP2080-500, ATGP2080-1

Osawa M. et al. (1997) Gene. 196:121-125.; ; Rizzu P. et al. (1995) Cytogenet Cell Genet.. 70:26-28.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl, 1mM DTT

Metabolism

NP_001613.1

P02765

Human

APHGPGL IYRQPNCDDP ETEEAALVAI DYINQNLPWG YKHTLNQIDE VKVWPQQPSG ELFEIEIDTL ETTCHVLDPT PVARCSVRQL KEHAVEGDCD FQLLKLDGKF SVVYAKCDSS PDSAEDVRKV CQDCPLLAPL NDTRVVHAAK AALAAFNAQN NGSNFQLEEI SRAQLVPLPP STYVEFTVSG TDCVAKEATE AAKCNLLAEK QYGFCKATLS EKLGGAEVAV TCTVFQTQPV TSQPQPEGAN EAVPTPVVDP DAPPSPPLGA PGLPPAGSPP DSHVLLAAPP GHQLHRAHYD LRHTFMGVVS LGSPSGEVSH PRKTRTVVQP SVGAAAGPVV PPCPGRIRHF KV