Recombinant humaan FGF-10-eiwit

FGF10, also known as fibroblast growth factor 10, exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. FGF10 is also implicated to be a primary factor in the process of wound healing. Recombinant human FGF10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Fibroblast growth factor 10, Keratinocyte growth factor 2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

22.0 kDa (196aa) confirmed by MALDI-TOF

FGF10, Fibroblast growth factor 10, Keratinocyte growth factor 2, ATGP1387-10 µg, ATGP1387-20 µg, ATGP1387-50 µg, ATGP1387-100 µg, ATGP1387-250 µg, ATGP1387-500 µg, ATGP1387-1 mg, ATGP1387-10, ATGP1387-20, ATGP1387-50, ATGP1387-100, ATGP1387-250, ATGP1387-500, ATGP1387-1

Entesarian M., et al. (2005) Nat. Genet. 37:125-127; ; Milunsky J.M., et al. (2006) Clin. Genet. 69:349-354

Jee JH, et al. Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture. (Stem Cells Int. 2019) {https://pubmed.ncbi.nlm.nih.gov/31312220/}; ; Park E, et al. Development of organoid-based drug metabolism model. (Toxicol Appl Pharmacol. 2019) {https://pubmed.ncbi.nlm.nih.gov/31678242/}; ; Kim HK, et al. Generation of tonsil organoids as an ex vivo model for SARS-CoV-2 infection. (bioRxiv. 2020) {https://doi.org/10.1101/2020.08.06.239574}; ; Kim HK, et al. Generation of human tonsil epithelial organoids as an ex vivo model for SARS-CoV-2 infection. (Biomaterials. 2022) {https://pubmed.ncbi.nlm.nih.gov/35286852/}; ; Go YH, et al. Modeling Pancreatic Cancer with Patient-Derived Organoids Integrating Cancer-Associated Fibroblasts. (Cancers (Basel) . 2022) {https://pubmed.ncbi.nlm.nih.gov/35565206/}; ; Lim JY, et al. Human Palatine Tonsils Are Linked to Alzheimers Disease through Function of Reservoir of Amyloid Beta Protein Associated with Bacterial Infection. (Cells. 2022) {https://pubmed.ncbi.nlm.nih.gov/35892582/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.2M NaCl, 2mM DTT, 2mM EDTA

Growth factors

NP_004456.1

O15520

Human

QALGQ DMVSPEATNS SSSSFSSPSS AGRHVRSYNH LQGDVRWRKL FSFTKYFLKI EKNGKVSGTK KENCPYSILE ITSVEIGVVA VKAINSNYYL AMNKKGKLYG SKEFNNDCKL KERIEENGYN TYASFNWQHN GRQMYVALNG KGAPRRGQKT RRKNTSAHFL PMVVHS