Recombinant humaan FGF zuur/FGF-1-eiwit

Acidic fibroblast growth factor (aFGF) belongs to the fibroblast growth factor family. It is a potent mitogenic agent. aFGF has been detected in large amounts in the brain. Other cells known to express FGF acidic include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. The growth factor acts through activation of specific cell-surface receptors leading to intracellular tyrosine phosphorylation cascade. Recombinant human aFGF was overexpressed in E. coli and purified by conventional chromatography.

Acidic Fibroblast Growth Factor, Fibroblast growth factor 1, Acidic fibroblast growth factor, aFGF, Endothelial cell growth factor, ECGF, Heparin-binding growth factor 1, HBGF-1, FGFA, ECGFA, ECGF-beta, FGF-alpha, GLIO703, ECGFB

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

15.9 kDa (141aa) confirmed by MALDI-TOF

FGF acidic, Acidic Fibroblast Growth Factor, ATGP3788-10 µg, ATGP3788-20 µg, ATGP3788-50 µg, ATGP3788-100 µg, ATGP3788-250 µg, ATGP3788-500 µg, ATGP3788-1 mg, ATGP3788-10, ATGP3788-20, ATGP3788-50, ATGP3788-100, ATGP3788-250, ATGP3788-500, ATGP3788-1

Jaye M. et al., (1986) Science. 233, 541-544.; ; Mergia A. et al., (1989) Biochem. Biophys. Res. Commun. 164, 1121-1129.; ; Stauber DJ. et al., (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 49-54.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.0) containing 100 mM NaCl

Hormones & Cytokines

NP_000791

P05230

Human

MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D