Recombinant humaan FHIT-eiwit

FHIT (fragile histidine triad) is an enzyme that cleaves adenosine 5' PPP 5' A to yield AMP and ADP. FHIT gene encompasses the common fragile site FRA3B on chromosome 3. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. This protein physically associates with ubiquitin conjugating enzyme 9. Recombinant human FHIT, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.

Bis (5'-adenosyl) -triphosphatase, AP3A hydrolase, AP3Aase, Adenosine 5'-monophosphoramidase FHIT, Adenylylsulfatase, Adenylylsulfate-ammonia adenylyltransferase, Diadenosine 5',5'''-P1, P3-triphosphate hydrolase, Dinucleosidetriphosphatase, Fragile histidine triad protein, FRA3B

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

17.9 kDa (155aa) confirmed by MALDI-TOF

FHIT, Fragile histidine triad protein, Bis (5'-adenosyl) -triphosphatase, AP3Aase, Fragile histidine triad protein AP3A hydrolase, AP3A hydrolase fragile site 3p14.2, Bis 5' adenosyl triphosphatase, Diadenosine 5' 5''' P1 P3 triphosphate hydrolase, Dinucleosidetriphosphatase, FRA 3B, FRA3B, Fragile histidine triad, Fragile histidine triad gene, Fragile histidine triad protein, Tumor suppressor protein., ATGP0563-10 µg, ATGP0563-20 µg, ATGP0563-50 µg, ATGP0563-100 µg, ATGP0563-250 µg, ATGP0563-500 µg, ATGP0563-1 mg, ATGP0563-10, ATGP0563-20, ATGP0563-50, ATGP0563-100, ATGP0563-250, ATGP0563-500, ATGP0563-1

Kmiec Z., et al. (2009) J Physiol Pharmacol. 60 (4) :63-70.; ; Ahmad F., et al. (2010) J Cancer Res Clin Oncol. 136 (3) :333-50.;

Kawaguchi M, et al. Synthesis of Fluorescent Probes Targeting Tumor-Suppressor Protein FHIT and Identification of Apoptosis-Inducing FHIT Inhibitors. (J Med Chem. 2021) {https://pubmed.ncbi.nlm.nih.gov/34160227/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Enzymes & Proteases

NP_002003

P49789

Human