Recombinant humaan fibrillarine/FBL-eiwit

FBL is a key small nucleolar protein in eukaryotes, which has an important role in pre-rRNA processing during ribosomal biogenesis. It is associated with the u3, u8, and u13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. This protein is a component of several ribonucleoproteins including a nucleolar small nuclear ribonucleoprotein (SnRNP) and one of the two classes of small nucleolar ribonucleoproteins (snoRNPs) . Recombinant human FBL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

RRNA 2'-O-methyltransferase fibrillarin, FIB, FLRN, RNu3IP1, rRNA 2'-O-methyltransferase fibrillarin, Nop1, 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, U6 snRNA 2'-O-methyltransferase fibrillarin

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

28.9 kDa (262aa), confirmed by MALDI-TOF

FBL, rRNA 2'-O-methyltransferase fibrillarin, FIB, FLRN, RNu3IP1, rRNA 2'-O-methyltransferase fibrillarin, ATGP0721-10 µg, ATGP0721-20 µg, ATGP0721-50 µg, ATGP0721-100 µg, ATGP0721-250 µg, ATGP0721-500 µg, ATGP0721-1 mg, ATGP0721-10, ATGP0721-20, ATGP0721-50, ATGP0721-100, ATGP0721-250, ATGP0721-500, ATGP0721-1

Amin MA, et al. (2007) Biochem Biophys Res Commun. 360 (2) :320-6; ; Jansen RP, et al. (1991) J Cell Biol 113 (4) : 715-29;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM EDTA

Enzymes & Proteases

NP_001427

P22087

Human

GKNVMVE PHRHEGVFIC RGKEDALVTK NLVPGESVYG EKRVSISEGD DKIEYRAWNP FRSKLAAAIL GGVDQIHIKP GAKVLYLGAA SGTTVSHVSD IVGPDGLVYA VEFSHRSGRD LINLAKKRTN IIPVIEDARH PHKYRMLIAM VDVIFADVAQ PDQTRIVALN AHTFLRNGGH FVISIKANCI DSTASAEAVF ASEVKKMQQE NMKPQEQLTL EPYERDHAVV VGVYRPPPKV KN