Recombinant humaan FIS1-eiwit

TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission. This protein can induce cytochrome C release from the mitochondrion to the cytosol, ultimately leading to apoptosis. It is also involved in peroxisomal growth and division. The C terminus is required for mitochondrial localisation, while the N teminus is necessary for mitochondrial fission. Recombinant human TTC11, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Mitochondrial fission 1 protein, FIS1 homolog, hFis1, Tetratricopeptide repeat protein 11, TPR repeat protein 11, TTC11, CGI-135 protein

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

16.3 kDa (142aa) confirmed by MALDI-TOF

CGI 135, CGI135, FIS 1, FIS1, Fis1 homolog, "Fission 1 (mitochondrial outer membrane) homolog", "Fission 1 (mitochondrial outer membrane) homolog (S, cerevisiae) ", "Fission 1 (mitochondrial outer membrane) homolog (yeast) Fission 1 homolog", H NH0132A01.6, hFis 1, hFis1, homolog of, Mitochondrial fission 1 protein, S. cerevisiae, "S, cerevisiae", Tetratricopeptide repeat domain 11, Tetratricopeptide repeat domain 11 2010003O14Rik, Tetratricopeptide repeat protein 11, TPR repeat protein 11, TTC 11, ATGP0562-10 µg, ATGP0562-20 µg, ATGP0562-50 µg, ATGP0562-100 µg, ATGP0562-250 µg, ATGP0562-500 µg, ATGP0562-1 mg, ATGP0562-010, ATGP0562-020, ATGP0562-050, ATGP0562-100, ATGP0562-250, ATGP0562-500, ATGP0562-01M

Martinou JC., et al. (2003) J Biol Chem. 278 (38) :36373-9.; ; Yoon Y., et al. (2008) Exp Cell Res. 314 (19) :3494-507 ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Apoptosis

NP_057152

Q9Y3D6

Human

MEAVLNELVS VEDLLKFEKK FQSEKAAGSV SKSTQFEYAW CLVRSKYNDD IRKGIVLLEE LLPKGSKEEQ RDYVFYLAVG NYRLKEYEKA LKYVRGLLQT EPQNNQAKEL ERLIDKAMKK DG