Recombinant humaan FKBP12,6/FKBP1B eiwit

FKBP1B, also known as peptidyl-prolyl cis-trans isomerase FKBP1B, is a member of the immunophilin protein family which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 (tacrolimus) and rapamycin (sirolimus) . It is highly similar to the FK506-binding protein 1A. Its physiological role is thought to be in excitation-contraction coupling in cardiac muscle. Recombinant human FKBP1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 800 nmol/min/mg, and is defined as the amount of enzyme cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

FKBP prolyl isomerase 1B, Peptidyl-prolyl cis-trans isomerase FKBP1B, PPIase FKBP1B, 12.6 kDa FK506-binding protein, 12.6 kDa FKBP, FKBP-12.6, FK506-binding protein 1B, FKBP-1B, Immunophilin FKBP12.6, Rotamase, h-FKBP-12, FKBP12.6, FKBP1L, FKBP9, OTK4, Calstabin 2

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 800 nmol/min/mg, and is defined as the amount of enzyme cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

14.1kDa (130aa) confirmed by MALDI-TOF

Rotamase, PPIase FKBP1B, PPIase, PKBP1L, Peptidyl-prolyl cis-trans isomerase FKBP1B, Peptidyl prolyl cis trans isomerase FKBP1B, OTK4, OTK 4, Immunophilin FKBP12.6, h-FKBP-12, h FKBP 12, FKBP9, FKBP1L, 12.6kDa FK506 binding protein, FKBP-1B, FKBP-12.6, FKBP1B, FKBP12.6, FKBP prolyl isomerase 1B, FKBP 1B, FKBP 12.6, 12.6 kDa FK506 binding protein, FK506-binding protein 1B, FK506 binding protein 1B, Calstabin 2, 12.6 kDa FKBP, 12.6 kDa FK506-binding protein, ATGP1266-10 µg, ATGP1266-20 µg, ATGP1266-50 µg, ATGP1266-100 µg, ATGP1266-250 µg, ATGP1266-500 µg, ATGP1266-1 mg, ATGP1266-010, ATGP1266-020, ATGP1266-050, ATGP1266-100, ATGP1266-250, ATGP1266-500, ATGP1266-01M

Arakawa H., et al. (1994) Biochem. Biophys. Res. 200:836-843; ; Deivanayagam C.C., et al. (2000) Acta Crystallogr. D 56:266-271

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Heat Shock Proteins

NP_004107

P68106

Human

MGVEIETI SPGDGRTFPK KGQTCVVHYT GMLQNGKKFD SSRDRNKPFK FRIGKQEVIK GFEEGAAQMS LGQRAKLTCT PDVAYGATGH PGVIPPNATL IFDVELLNLE