Recombinant humaan FKBP12/FKBP1A-eiwit

FK506 binding protein 1a, also known as FKBP12, belongs to the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1, 4, 5-trisphosphate receptor. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. Recombinant human FKBP12, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 800 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

FKBP prolyl isomerase 1A, FKBP1, FK506 binding protein 1a 12kDa, PPIase FKBP1A, Rotamase, 12 kDa FKBP, FK506 binding protein 1, FK506 binding protein 1A 12kDa, Immunophilin FKBP12, FK506 binding protein T cell 12 kD, FK506 binding protein 12, FKBP 12, FKBP12 Exip3, FKBP12C, Peptidyl-prolyl cis-trans isomerase, PKCI2, PPIase, Protein kinase C inhibitor 2, calstabin 1, Rotamase

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 800 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

14.1 kDa (128aa) confirmed by MALDI-TOF

FKBP1a , FKBP12, FK506 binding protein 1a 12kDa, PPIase FKBP1A, Rotamase, FKBP1, PKC12, FK506 binding protein 1a, 12kDa 12 kDa FKBP, FK506 binding protein 1, FK506 binding protein 1A 12kDa, Immunophilin FKBP12, FK506 binding protein T cell 12 kD, FK506-binding protein 1A, FK506 binding protein 12, FKBP 12, FKBP 1A, FKBP12, FKBP12 Exip3, FKBP12C, Peptidyl-prolyl cis-trans isomerase, PKCI2, PPIase, Protein kinase C inhibitor 2., ATGP0290-10 µg, ATGP0290-20 µg, ATGP0290-50 µg, ATGP0290-100 µg, ATGP0290-250 µg, ATGP0290-500 µg, ATGP0290-1 mg, ATGP0290-10, ATGP0290-20, ATGP0290-50, ATGP0290-100, ATGP0290-250, ATGP0290-500, ATGP0290-1

Jayaraman T., et al. (1992) . J Biol Chem. 267 (14) :9474-7; ; Siekierka JJ., et al. (1990) . J Biol Chem. 265 (34) :21011-5;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 1mM DTT, 10% glycerol

Heat Shock Proteins

NP_463460.1

P62942

Human

MGVQVETISP GDGRTFPKRG QTCVVHYTGM LEDGKKFDSS RDRNKPFKFM LGKQEVIRGW EEGVAQMSVG QRAKLTISPD YAYGATGHPG IIPPHATLVF DVELLKLE