Recombinant humaan FKBP13/FKBP2-eiwit

FKBP2 is peptidyl proline isomerase enzyme and a member of the immunophilin protein family. This protein is predominantly expressed in thymus and T cells and plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. FKBP2 is thought to function as an ER chaperone and may also act as a component of membrane cytoskeletal scaffolds. Recombinant FKBP2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

FKBP prolyl isomerase 2, Peptidyl-prolyl cis-trans isomerase FKBP2, PPIase FKBP2, 13 kDa FK506-binding protein, 13 kDa FKBP, FKBP-13, FK506-binding protein 2, FKBP-2, Immunophilin FKBP13, Rotamase, Rapamycin-binding protein, Proline isomerase

E.coli

Human

Non-Tagged

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

13.4 kDa (122aa) confirmed by MALDI-TOF

Rotamase, Rapamycin-binding protein, Rapamycin binding protein, Proline isomerase, PPIase FKBP2, PPIase, Peptidyl-prolyl cis-trans isomerase FKBP2 13 kDa FKBP, Peptidyl-prolyl cis-trans isomerase FKBP2, Peptidyl prolyl cis trans isomerase FKBP2 13kDa FKBP, Peptidyl prolyl cis trans isomerase FKBP2, Peptidyl prolyl cis trans isomerase, Immunophilin FKBP13, FKBP-2, FKBP2, FKBP-13, FKBP13, FKBP prolyl isomerase 2, FKBP 2, FKBP 13, FK506-binding protein 2, FK506 binding protein 2 13kDa, FK506 binding protein 2 13kD, FK506 binding protein 2, EC 5.2.1.8, 13kDa FKBP, 13kDa FK506 binding protein, 13 kDa FKBP, 13 kDa FK506-binding protein, 13 kDa FK506 binding protein, ATGP0506-10 µg, ATGP0506-20 µg, ATGP0506-50 µg, ATGP0506-100 µg, ATGP0506-250 µg, ATGP0506-500 µg, ATGP0506-1 mg, ATGP0506-010, ATGP0506-020, ATGP0506-050, ATGP0506-100, ATGP0506-250, ATGP0506-500, ATGP0506-01M

Walensky LD., et al. (1998) J Cell Biol. 141 (1) :143-53. ; ; Padilla PI., et al. (2003) Proc Natl Acad Sci u S A. 100 (5) :2322-7.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Heat Shock Proteins

NP_001128680

P26885

Human

MATGAEGKRK LQIGVKKRVD HCPIKSRKGD VLHMHYTGKL EDGTEFDSSL PQNQPFVFSL GTGQVIKGWD QGLLGMCEGE KRKLVIPSEL GYGERGAPPK IPGGATLVFE VELLKIERRT EL