Recombinant humaan FKBP22/FKBP14-eiwit

FKBP14, also known as 22 kDa FK506-binding protein, is an enzyme that accelerates the folding of proteins during protein synthesis. This protein contains two EF-hand domains and one PPIase FKBP-type domain. Truncation of the amino-terminus of FKBP14 greatly reduces peptidyl prolyl cis-trans isomerase activity, therefore suggesting that the PPIase FKBP-type domain must be located at the N-terminus. Recombinant human FKBP14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography

Specific activity is > 240 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin.

22 kDa FK506 binding protein, FK506 binding protein 14 (22 kDa), FKBP22, Peptidyl prolyl cis trans isomerase, Peptidyl-prolyl cis-trans isomerase FKBP14, PPIase, Rotamase

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 240 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin.

24.2 kDa (213aa) confirmed by MALDI-TOF

22 kDa FK506 binding protein, FK506 binding protein 14, FK506 binding protein 14 (22 kDa), FKBP 22, FKBP22, Peptidyl prolyl cis trans isomerase, Peptidyl-prolyl cis-trans isomerase FKBP14, PPIase, Rotamase, FKBP14, FKBP 14, ATGP0604-10 µg, ATGP0604-20 µg, ATGP0604-50 µg, ATGP0604-100 µg, ATGP0604-250 µg, ATGP0604-500 µg, ATGP0604-1 mg, ATGP0604-010, ATGP0604-020, ATGP0604-050, ATGP0604-100, ATGP0604-250, ATGP0604-500, ATGP0604-01M

Tremmel D., et al. (2007) J Mol Biol. 369 (1) :55-68.; ; Budiman C., et al. (2009) FEBS J. 276 (15) :4091-101.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Heat Shock Proteins

NP_060416

Q9NWM8

Human

MGSSHHHHHH SSGLVPRGSH MALIPEPEVK IEVLQKPFIC HRKTKGGDLM LVHYEGYLEK DGSLFHSTHK HNNGQPIWFT LGILEALKGW DQGLKGMCVG EKRKLIIPPA LGYGKEGKGK IPPESTLIFN IDLLEIRNGP RSHESFQEMD LNDDWKLSKD EVKAYLKKEF EKHGAVVNES HHDALVEDIF DKEDEDKDGF ISAREFTYKH DEL