Recombinant humaan FKBP25/FKBP3-eiwit

FK506 binding protein 3 (FKBP3), also known as FKBP25, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP3 associates with transcriptional repressor protein YY1 and histone deaceltylases, HDAC1 and HDAC2. Also, FKBP3 may contain several casein kinase II phosphorylation sites, which are believed to be important for cell growth regulation. It is localized in the nucleus and is expressed in the brain, testis, ovary, and spleen. Recombinant human FKBP3 was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 900 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

FKBP prolyl isomerase 3, Peptidyl-prolyl cis-trans isomerase FKBP3, PPIase FKBP3, 25 kDa FK506-binding protein, 25 kDa FKBP, FKBP-25, FK506-binding protein 3, FKBP-3, Immunophilin FKBP25, Rapamycin-selective 25 kDa immunophilin, Rotamase

E.coli

Human

Non-Tagged

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 900 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

25.1 kDa (224aa) confirmed by MALDI-TOF

Rotamase, Rapamycin-selective 25kDa immunophilin, Rapamycin-selective 25 kDa immunophilin, Rapamycin selective 25kDa immunophilin, Rapamycin selective 25 kDa immunophilin, PPIase FKBP3, PPIase, Peptidyl-prolyl cis-trans isomerase FKBP3, Peptidyl prolyl cis trans isomerase FKBP3, Immunophilin FKBP25, FKBP-3, FKBP3, FKBP-25, FKBP25, FKBP prolyl isomerase 3, FKBP 3, FKBP 25, FK506-binding protein 3, FK506 binding protein3, FK506 binding protein 3, 25kDa FKBP, 25kDa FK506 binding protein, 25 kDa FKBP, 25 kDa FK506-binding protein, 25 kDa FK506 binding protein, ATGP0494-10 µg, ATGP0494-20 µg, ATGP0494-50 µg, ATGP0494-100 µg, ATGP0494-250 µg, ATGP0494-500 µg, ATGP0494-1 mg, ATGP0494-010, ATGP0494-020, ATGP0494-050, ATGP0494-100, ATGP0494-250, ATGP0494-500, ATGP0494-01M

Yang WM., et al. (2001) EMBO J. 20 (17) :4814-25. ; ; Jin YJ., et al. (1993) Proc Natl Acad Sci u S A. 90 (16) :7769-73. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Heat Shock Proteins

NP_002004

Q00688

Human

MAAAVPQRAW TVEQLRSEQL PKKDIIKFLQ EHGSDSFLAE HKLLGNIKNV AKTANKDHLV TAYNHLFETK RFKGTESISK VSEQVKNVKL NEDKPKETKS EETLDEGPPK YTKSVLKKGD KTNFPKKGDV VHCWYTGTLQ DGTVFDTNIQ TSAKKKKNAK PLSFKVGVGK VIRGWDEALL TMSKGEKARL EIEPEWAYGK KGQPDAKIPP NAKLTFEVEL VDID