Recombinant humaan FLIP-eiwit

CFLAR, also known as FLIP, is involved in the regulation of apoptosis. It has been designated by independent groups as Casper, I-FLICE, CLARP, FLAME-1 and MRIT. Although its exact role is still being elucidated, it appears to be an important factor in the regulation of apoptosis downstream of all known death receptors. Recombinant human CFLAR protein was expressed in E. coli and denatured using detergent during a conventional chromatography purification process.

CASP8 and FADD-like apoptosis regulator, CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, uSuRPIN

E.coli

Human

Non-Tagged

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

55.3 kDa (480aa)

FLIP, CASP8 and FADD-like apoptosis regulator, CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, uSuRPIN, ATGP1195-10 µg, ATGP1195-20 µg, ATGP1195-50 µg, ATGP1195-100 µg, ATGP1195-250 µg, ATGP1195-500 µg, ATGP1195-1 mg, ATGP1195-10, ATGP1195-20, ATGP1195-50, ATGP1195-100, ATGP1195-250, ATGP1195-500, ATGP1195-1

Hu S. et al. (1997) J Biol Chem. 272: 17255-17257.; ; Thome M. et al. (1997) . Nature 386: 517-521

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Apoptosis

NP_003870

O15519

Human

MSAEVIHQVE EALDTDEKEM LLFLCRDVAI DVVPPNVRDL LDILRERGKL SVGDLAELLY RVRRFDLLKR ILKMDRKAVE THLLRNPHLV SDYRVLMAEI GEDLDKSDVS SLIFLMKDYM GRGKISKEKS FLDLVVELEK LNLVAPDQLD LLEKCLKNIH RIDLKTKIQK YKQSVQGAGT SYRNVLQAAI QKSLKDPSNN FRLHNGRSKE QRLKEQLGAQ QEPVKKSIQE SEAFLPQSIP EERYKMKSKP LGICLIIDCI GNETELLRDT FTSLGYEVQK FLHLSMHGIS QILGQFACMP EHRDYDSFVC VLVSRGGSQS VYGVDQTHSG LPLHHIRRMF MGDSCPYLAG KPKMFFIQNY VVSEGQLENS SLLEVDGPAM KNVEFKAQKR GLCTVHREAD FFWSLCTADM SLLEQSHSSP SLYLQCLSQK LRQERKRPLL DLHIELNGYM YDWNSRVSAK EKYYVWLQHT LRKKLILSYT