Recombinant humaan FN3KRP-eiwit

Ketosamine-3-kinase, also known as FN3KRP, catalyzes the phosphorylation of psicosamines and ribulosamines compared to the neighboring gene which encodes a highly similar enzyme, fructosamine-3-kinase, which has different substrate specificity. The activity of both enzymes may result in deglycation of proteins to restore their function. Recombinant human FN3KRP protein, fused to His-tag at N-terminus, was expressed in E. coli.

Ketosamine-3-kinase, FN3KL, FN3K-related protein

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36.8 kDa (332aa)

FN3KRP, Ketosamine-3-kinase, FN3KL, FN3K-related protein, ATGP1892-10 µg, ATGP1892-20 µg, ATGP1892-50 µg, ATGP1892-100 µg, ATGP1892-250 µg, ATGP1892-500 µg, ATGP1892-1 mg, ATGP1892-10, ATGP1892-20, ATGP1892-50, ATGP1892-100, ATGP1892-250, ATGP1892-500, ATGP1892-1

Collard F., et al. (2004) Biochem. J. 382:137-143; ; Oppermann F.S., et al. (2009) Mol. Cell. Proteomics. 8:1751-1764

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Metabolism

NP_078895

Q9HA64

Human

MEELLRR ELGCSSVRAT GHSGGGCISQ GRSYDTDQGR VFVKVNPKAE ARRMFEGEMA SLTAILKTNT VKVPKPIKVL DAPGGGSVLV MEHMDMRHLS SHAAKLGAQL ADLHLDNKKL GEMRLKEAGT VGRGGGQEER PFVARFGFDV VTCCGYLPQV NDWQEDWVVF YARQRIQPQM DMVEKESGDR EALQLWSALQ LKIPDLFRDL EIIPALLHGD LWGGNVAEDS SGPVIFDPAS FYGHSEYELA IAGMFGGFSS SFYSAYHGKI PKAPGFEKRL QLYQLFHYLN HWNHFGSGYR GSSLNIMRNL VK