Recombinant humaan Follistatine/FST-eiwit

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. Recombinant human FST protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Follistatin, Follistatin, FS, FST344

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

37 kDa (336aa) confirmed by MALDI-TOF

FST, Follistatin, Follistatin, FS, ATGP2017-10 µg, ATGP2017-20 µg, ATGP2017-50 µg, ATGP2017-100 µg, ATGP2017-250 µg, ATGP2017-500 µg, ATGP2017-1 mg, ATGP2017-10, ATGP2017-20, ATGP2017-50, ATGP2017-100, ATGP2017-250, ATGP2017-500, ATGP2017-1

Lerch T.F., et al. (2007) J. Biol. Chem. 282:15930-15939; ; Cash J.N., et al. (2009) EMBO J. 28:2662-2676;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Signal Transduction

NP_037541

P19883

Human

MGNCWLRQAK NGRCQVLYKT ELSKEECCST GRLSTSWTEE DVNDNTLFKW MIFNGGAPNC IPCKETCENV DCGPGKKCRM NKKNKPRCVC APDCSNITWK GPVCGLDGKT YRNECALLKA RCKEQPELEV QYQGRCKKTC RDVFCPGSST CVVDQTNNAY CVTCNRICPE PASSEQYLCG NDGVTYSSAC HLRKATCLLG RSIGLAYEGK CIKAKSCEDI QCTGGKKCLW DFKVGRGRCS LCDELCPDSK SDEPVCASDN ATYASECAMK EAACSSGVLL EVKHSGSCNS ISEDTEEEEE DEDQDYSFPI SSILEW