Recombinant humaan Follistatine/FST-eiwit

FST, also known as Follistatin, is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. Recombinant human FST, fused to His-tag at C-terminus, was expressed in Sf9 and purified by using conventional chromatography techniques.

Follistatin isoform FST317, FS

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

32.5 kDa (295aa) 40-57 kDa, reducing conditions

Follistatin (aa300344), Follistatin isoform FST344, FS, ATGP2964-10 µg, ATGP2964-20 µg, ATGP2964-50 µg, ATGP2964-100 µg, ATGP2964-250 µg, ATGP2964-500 µg, ATGP2964-1 mg, ATGP2964-10, ATGP2964-20, ATGP2964-50, ATGP2964-100, ATGP2964-250, ATGP2964-500, ATGP2964-1

Sang Q, Zhang S, et al. (2013) . Reprod Biomed Online. 26 (2) :157-63.; ; Akiyama I, Yoshino O, et al. (2013) . Reprod Sci. 20 (6) :675-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_006341

P19883

Human

MGNCWLRQAK NGRCQVLYKT ELSKEECCST GRLSTSWTEE DVNDNTLFKW MIFNGGAPNC IPCKETCENV DCGPGKKCRM NKKNKPRCVC APDCSNITWK GPVCGLDGKT YRNECALLKA RCKEQPELEV QYQGRCKKTC RDVFCPGSST CVVDQTNNAY CVTCNRICPE PASSEQYLCG NDGVTYSSAC HLRKATCLLG RSIGLAYEGK CIKAKSCEDI QCTGGKKCLW DFKVGRGRCS LCDELCPDSK SDEPVCASDN ATYASECAMK EAACSSGVLL EVKHSGSCNH HHHHH