Recombinant humaan FOLR2-eiwit

FOLR2, also known as Folate receptor beta, is a member of the folate receptor (FLOR) family. Members of this gene family have a high affinity for folate and folic acid analogs at neutral pH. And they mediate delivery of 5-methyltetrahydrofolate to the interior of cells. It was originally thought to exist only in placenta, but is also expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells. FOLR2 is also upregulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma. Recombinant human FOLR2, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Folic Acid-BSA is coated at 10 µg/mL (100 ul/well) can bind Human FOLR2. The ED50 range ≤ 5 µg/mL.

Folate receptor beta, FR-beta, Folate receptor 2, Folate receptor, fetal/placental, Placental folate-binding protein, FBP, FBP Protein, BETA-HFR Protein, FBP/PL-1 Protein, FR-BETA Protein, FR-P3 Protein, FRbeta, FR-BETA, FBP/PL-1, BETA-HFR

HEK293

Human

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Folic Acid-BSA. The ED50 range ≤ 5 µg/mL.

25.1 kDa (215aa)

, ATGP3987-10 µg, ATGP3987-20 µg, ATGP3987-50 µg, ATGP3987-100 µg, ATGP3987-250 µg, ATGP3987-500 µg, ATGP3987-1 mg, ATGP3987-10, ATGP3987-20, ATGP3987-50, ATGP3987-100, ATGP3987-250, ATGP3987-500, ATGP3987-1

Ross, J.F. et al.,1994, Cancer 73:2432.; ; Joost W van der Heijden J.W. et al., 2009, Arthritis Rheum. 60:12-21.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction, Metabolism

NP_000794.3

P14207

Human

Gln 22