Recombinant humaan Frataxin-eiwit

Frataxin, also known as FXN, is an iron binding protein which belongs to FRATAXIN family. It is an anti-apoptotic protein which prevents mitochondrial damage, reactive oxygen species production and act as either an iron chaperone or an iron storage protein. Frataxin is localized to the mitochondrion and regulating mitochondrial iron transport and respiration. Defects in this protein cause Friedreich ataxia. Recombinant Frataxin protein was expressed in E. coli and purified by using conventional chromatography techniques.

CyaY, FA, FARR, FRDA, X25, FXN, Friedreich ataxia protein.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

21.1 kDa (190aa) confirmed by MALDI-TOF

Frataxin, ATGP0448-10 µg, ATGP0448-20 µg, ATGP0448-50 µg, ATGP0448-100 µg, ATGP0448-250 µg, ATGP0448-500 µg, ATGP0448-1 mg, ATGP0448-10, ATGP0448-20, ATGP0448-50, ATGP0448-100, ATGP0448-250, ATGP0448-500, ATGP0448-1

Campuzano V., et al. (1996) Science. 271 (5254) :1423-7. ; ; Shan Y., et al. (2007) Hum Mol Genet. 16 (8) :929-41. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 20% glycerol, 0.1M NaCl 1mM DTT

Apoptosis

NP_000135.2

Q16595

Human

LRTDIDATC TPRRASSNQR GLNQIWNVKK QSVYLMNLRK SGTLGHPGSL DETTYERLAE ETLDSLAEFF EDLADKPYTF EDYDVSFGSG VLTVKLGGDL GTYVINKQTP NKQIWLSSPS SGPKRYDWTG KNWVYSHDGV SLHELLAAEL TKALKTKLDL SSLAYSGKDA