Recombinant humaan Fructosamine-3-kinase/FN3K-eiwit

FN3K catalyzes the phosphorylation of fructosamines which may result in deglycation, the non-enzymatic reaction of glucose with primary amines followed by Amadori re-arrangement. Phosphorylation of fructosamines may initiate metabolism of the modified amine and lead to the de-glycation of fructoselysine and of glycated proteins. Recombinant human FN3K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Fructosamine-3-kinase, Fructosamine 3 kinase

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

37 kDa (332aa) confirmed by MALDI-TOF

Fructosamine3-kinase, FN3K, Fructosamine-3-kinase, Fructosamine 3 kinase, ATGP1884-10 µg, ATGP1884-20 µg, ATGP1884-50 µg, ATGP1884-100 µg, ATGP1884-250 µg, ATGP1884-500 µg, ATGP1884-1 mg, ATGP1884-10, ATGP1884-20, ATGP1884-50, ATGP1884-100, ATGP1884-250, ATGP1884-500, ATGP1884-1

Conner JR, Beisswenger PJ, et al. (2005) . Ann N Y Acad Sci. 1043:824-36.; ; Delpierre G, Veiga-da-Cunha M, et al. (2006) . Diabetes Metab. 32 (1) :31-9.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Metabolism

NP_071441.1

Q9H479

Human

MEQLLRA ELRTATLRAF GGPGAGCISE GRAYDTDAGP VFVKVNRRTQ ARQMFEGEVA SLEALRSTGL VRVPRPMKVI DLPGGGAAFV MEHLKMKSLS SQASKLGEQM ADLHLYNQKL REKLKEEENT VGRRGEGAEP QYVDKFGFHT VTCCGFIPQV NEWQDDWPTF FARHRLQAQL DLIEKDYADR EARELWSRLQ VKIPDLFCGL EIVPALLHGD LWSGNVAEDD VGPIIYDPAS FYGHSEFELA IALMFGGFPR SFFTAYHRKI PKAPGFDQRL LLYQLFNYLN HWNHFGREYR SPSLGTMRRL LK