Recombinant humaan FUBP1-eiwit

FUBP1 is a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase. Recombinant human FUBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Far upstream element (FuSE) binding protein 1, FBP, FuBP

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by BCA assay)

> 85% by SDS-PAGE

20.8 kDa (195aa) confirmed by MALDI-TOF

FUBP1, Far upstream element (FuSE) binding protein 1, FBP, FuBP, ATGP1587-10 µg, ATGP1587-20 µg, ATGP1587-50 µg, ATGP1587-100 µg, ATGP1587-250 µg, ATGP1587-500 µg, ATGP1587-1 mg, ATGP1587-10, ATGP1587-20, ATGP1587-50, ATGP1587-100, ATGP1587-250, ATGP1587-500, ATGP1587-1

Zheng, Y., et al. (2011) Int. J. Biochem. Cell Biol. 43 (11), 1641-1648; ; Neubauer, A., et al. (2011) Exp. Hematol. 39 (10), 1030-1042.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl

Epigenomics (Transcription & Translation)

NP_003893.2

Q96AE4

Human

DVPIP RFAVGIVIGR NGEMIKKIQN DAGVRIQFKP DDGTTPERIA QITGPPDRCQ HAAEIITDLL RSVQAGNPGG PGPGGRGRGR GQGNWNMGPP GGLQEFNFIV PTGKTGLIIG KGGETIKSIS QQSGARIELQ RNPPPNADPN MKLFTIRGTP QQIDYARQLI EEKIG