Recombinant humaan Fucosyltransferase 3/FUT3-eiwit

Fucosyltransferase 3, also known as FuT3, may catalyze alpha-1, 3 and alpha-1, 4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. This protein may be involved in blood group Lewis determination; Lewis-positive (Le+) individuals have an active enzyme while Lewis-negative (Le-) individuals have an inactive enzyme. Also acts on the corresponding 1, 4-galactosyl derivative, forming 1, 3-L-fucosyl links. Recombinant human FuT3 protein, fused to His-tag at N-terminus, was expressed in E. coli.

3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, Alpha-3-fucosyltransferase FUT3, Blood group Lewis alpha-4-fucosyltransferase, Lewis FT, Fucosyltransferase 3, Fucosyltransferase III, FucT-III, FT3B, LE

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

40.6 kDa (350aa)

Fucosyltransferase3, FUT3, Fucosyltransferase 3, Fucosyltransferase 3, CD174, FT3B, FucT-III, LE, Les, ATGP2322-10 µg, ATGP2322-20 µg, ATGP2322-50 µg, ATGP2322-100 µg, ATGP2322-250 µg, ATGP2322-500 µg, ATGP2322-1 mg, ATGP2322-10, ATGP2322-20, ATGP2322-50, ATGP2322-100, ATGP2322-250, ATGP2322-500, ATGP2322-1

Cameron H.S., et al. (1995) J. Biol. Chem. 270:20112-20122; ; Nishihara S., et al. (1993) Biochem. Biophys. Res. Commun. 196:624-631;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Cardiovascular

NP_001091110

P21217

Human

RVSRDDA TGSPRAPSGS SRQDTTPTRP TLLILLWTWP FHIPVALSRC SEMVPGTADC HITADRKVYP QADTVIVHHW DIMSNPKSRL PPSPRPQGQR WIWFNLEPPP NCQHLEALDR YFNLTMSYRS DSDIFTPYGW LEPWSGQPAH PPLNLSAKTE LVAWAVSNWK PDSARVRYYQ SLQAHLKVDV YGRSHKPLPK GTMMETLSRY KFYLAFENSL HPDYITEKLW RNALEAWAVP VVLGPSRSNY ERFLPPDAFI HVDDFQSPKD LARYLQELDK DHARYLSYFR WRETLRPRSF SWALDFCKAC WKLQQESRYQ TVRSIAAWFT