Recombinant humaan FUS2/NAA80-eiwit

N-acetyltransferase, also known as FuS2 (NAT6), is an enzyme that catalyzes the transfer of acetyl groups from acetyl-CoA to acrylamines. This enzyme is physically localized in the cytoplasm and its activity has been documented by its feasibility to acetylate the N-terminus of proteins using a ping-pong-like mechanism and by its substrate specificity. Since the Fus-2 gene maps to the chromosomal region 3p21. 3, which contains at least one tumor suppressor gene, the N-acetyltransferase functions of Fus-2 may be relevant to its potential role in cancer. Recombinant human N-acetyltransferase 6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Protein fus 2, Protein fusion-2, FuS-2, NAT6, N acetyltransferase 6

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

35.9 kDa (328aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Protein fusion-2, Protein fusion 2, Protein fusion2, Protein fus 2, NatH catalytic subunit, NAT6, N-alpha-acetyltransferase 80, NAA80, N acetyltransferase 6, FuS-2, FuS2, FuS 2, ATGP0285-10 µg, ATGP0285-20 µg, ATGP0285-50 µg, ATGP0285-100 µg, ATGP0285-250 µg, ATGP0285-500 µg, ATGP0285-1 mg, ATGP0285-010, ATGP0285-020, ATGP0285-050, ATGP0285-100, ATGP0285-250, ATGP0285-500, ATGP0285-01M

Zegerman P., et al. (2000) . Oncogene. 19 (1) :161-3.; ; Gatphayak K., et al. (2004) . Gene. 337:105-11;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM MES buffer (pH 5.0) containing 150 mM NaCl, 30% glycerol

Enzymes & Proteases

NP_036323

Q93015

Human

MQELTLSPGP AKLTPTLDPT HRMELILSTS PAELTLDPAC QPKLPLDSTC QPEMTFNPGP TELTLDPEHQ PEETPAPSLA ELTLEPVHRR PELLDACADL INDQWPRSRT SRLHSLGQSS DAFPLCLMLL SPHPTLEAAP VVVGHARLSR VLNQPQSLLV ETVVVARALR GRGFGRRLME GLEVFARARG FRKLHLTTHD QVHFYTHLGY QLGEPVQGLV FTSRRLPATL LNAFPTAPSP RPPRKAPNLT AQAAPRGPKG PPLPPPPPLP ECLTISPPVP SGPPSKSLLE TQYQNVRGRP IFWMEKDI