Recombinant humaan G3BP1-eiwit

G3BP1 is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. And it cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-uTR. Recombinant human G3BP1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

G3BP stress granule assembly factor 1, GTPase activating protein (SH3 domain) binding protein 1, Ras-GTPase-activating protein SH3-domain-binding protein, DNA helicase VIII, ATP-dependent DNA helicase VIII, GAP SH3 domain-binding protein 1, DH-VIII, G3BP, G3BP-1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

53.2 kDa (474aa) confirmed by MALDI-TOF

Ras-GTPase-activating protein SH3-domain-binding protein, RasGAP associated endoribonuclease G3BP, Ras GTPase-activating protein-binding protein 1, Ras GTPase activating protein SH3 domain binding protein, Ras GTPase activating protein binding protein 1, MGC111040, HDH VIII, GTPase activating protein binding protein 1, GTPase activating protein (SH3 domain) binding protein 1, GAP SH3 domain-binding protein 1, GAP SH3 domain binding protein 1, GAP binding protein, G3BP-1, G3BP1, G3BP stress granule assembly factor 1, G3BP 1, G3BP, DNA helicase VIII, DH-VIII, DHVIII, DH VIII, ATP-dependent DNA helicase VIII, ATP dependent DNA helicase VIII, ATGP0841-10 µg, ATGP0841-20 µg, ATGP0841-50 µg, ATGP0841-100 µg, ATGP0841-250 µg, ATGP0841-500 µg, ATGP0841-1 mg, ATGP0841-010, ATGP0841-020, ATGP0841-050, ATGP0841-100, ATGP0841-250, ATGP0841-500, ATGP0841-01M

Kurz, J.C., et al. (2000) Curr. Opin. Chem. Biol. 4: 553-558.; ; van Eenennaam, H., et al. (2001) Mol. Biol. Cell. 12: 3680-3689.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100 mM NaCl

Signal Transduction

NP_005745.1

Q13283

Human