Recombinant humaan GAD1/GAD67-eiwit

GAD1 is one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. It is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This protien may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. Recombinant human GAD1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Glutamate decarboxylase 1 isoform GAD25, Glutamate decarboxylase 1 (brain, 67kDa), CPSQ1, GAD, SCP

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

27.7 kDa (247aa)

GAD1, GAD67, Glutamate decarboxylase 1 isoform GAD25, Glutamate decarboxylase 1 (brain, 67kDa), CPSQ1, GAD, SCP, ATGP2642-10 µg, ATGP2642-20 µg, ATGP2642-50 µg, ATGP2642-100 µg, ATGP2642-250 µg, ATGP2642-500 µg, ATGP2642-1 mg, ATGP2642-10, ATGP2642-20, ATGP2642-50, ATGP2642-100, ATGP2642-250, ATGP2642-500, ATGP2642-1

Chessler S.D., Lernmark A., et al. (2000) J. Biol. Chem. 275:5188-5192.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Neuroscience

NP_038473.2

Q99259

Human

MASSTPS SSATSSNAGA DPNTTNLRPT TYDTWCGVAH GCTRKLGLKI CGFLQRTNSL EEKSRLVSAF KERQSSKNLL SCENSDRDAR FRRTETDFSN LFARDLLPAK NGEEQTVQFL LEVVDILLNY VRKTFDRSTK VLDFHHPHQL LEGMEGFNLE LSDHPESLEQ ILVDCRDTLK YGVRTGHPRF FNQLSTGLDI IGLAGEWLTS TANTNMPSDM RECWLLR