Recombinant humaan GADD45 alfa-eiwit

GADD45A, also known as growth arrest and DNA damage-inducible protein, binds both Cdks and PCNA, a protein involved in DNA replication and repair. It has been shown to stimulate DNA excision repair in vitro and to inhibit entry of cells into S phase. This protein may serve as a link between p53-dependent cell cycle checkpoint and DNA repair. Recombinant human GADD45A protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Growth arrest and DNA damage-inducible protein GADD45 alpha, DDIT1, GADD45, Growth arrest and DNA damage-inducible protein GADD45 alpha DDIT 1, DNA damage inducible transcript 1, Growth arrest and DNA damage inducible 45 alpha

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

19.4 kDa (173aa) confirmed by MALDI-TOF

GADD45 a, Growth arrest and DNA damage-inducible protein GADD45 alpha, DDIT1, GADD45, Growth arrest and DNA damage-inducible protein GADD45 alpha DDIT 1, DNA damage inducible transcript 1, Growth arrest and DNA damage inducible 45 alpha, ATGP0548-10 µg, ATGP0548-20 µg, ATGP0548-50 µg, ATGP0548-100 µg, ATGP0548-250 µg, ATGP0548-500 µg, ATGP0548-1 mg, ATGP0548-10, ATGP0548-20, ATGP0548-50, ATGP0548-100, ATGP0548-250, ATGP0548-500, ATGP0548-1

Hollander MC. et al. (1993) J Biol Chem. 268 (32) :24385-93; ; Takekawa M. et al. (1998) Cell. 95 (4) :521-30.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl

Cell Cycle

NP_001915

P24522

Human