Recombinant humaan GAGE12F eiwit

GAGE12F belongs to a multigene family expressed in a large variety of tumors whereas in normal tissues, expression is restricted to germ cells. These genes organized in clustered repeats, have a high degree of predicted sequence identity, but differ by scattered single nucleotide substitution. Their sequences contain either the antigenic peptide YYWPRPRRY or YRPRPRRY which is recognized by cytotoxic T-cells. Recombinant human GAGE12F protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

G antigen 12F, OTTHuMG00000024147

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by BCA assay)

> 85% by SDS-PAGE

15.4 kDa (140aa) confirmed by MALDI-TOF

GAGE12F, G antigen 12F, OTTHuMG00000024147, ATGP2387-10 µg, ATGP2387-20 µg, ATGP2387-50 µg, ATGP2387-100 µg, ATGP2387-250 µg, ATGP2387-500 µg, ATGP2387-1 mg, ATGP2387-10, ATGP2387-20, ATGP2387-50, ATGP2387-100, ATGP2387-250, ATGP2387-500, ATGP2387-1

Gjerstorff M.F., et al (2008) . Tissue Antigens 71:187-192; ; The MGC Project Team (2004) Genome Res. 14:2121-2127;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Cancer

NP_001091875

O76087

Human

MSWRGRS TYYWPRPRRY VQPPEMIGPM RPEQFSDEVE PATPEEGEPA TQRQDPAAAQ EGEDEGASAG QGPKPEAHSQ EQGHPQTGCE CEDGPDGQEM DPPNPEEVKT PEEGEKQSQC