Recombinant humaan GAGE2A-eiwit

GAGE2A belongs to a multigene family expressed in a large variety of tumors whereas in normal tissues, expression is restricted to germ cells. These genes organized in clustered repeats, have a high degree of predicted sequence identity, but differ by scattered single nucleotide substitution. Their sequences contain either the antigenic peptide YYWPRPRRY or YRPRPRRY which is recognized by cytotoxic T-cells. Recombinant human GAGE2A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

G antigen 2A/2B, CT4.2, GAGE-2, GAGE-2A, GAGE2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

15.2 kDa (139aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

GAGE2A, G antigen 2A/2B, CT4.2, GAGE-2, GAGE-2A, GAGE2, ATGP2337-10 µg, ATGP2337-20 µg, ATGP2337-50 µg, ATGP2337-100 µg, ATGP2337-250 µg, ATGP2337-500 µg, ATGP2337-1 mg, ATGP2337-10, ATGP2337-20, ATGP2337-50, ATGP2337-100, ATGP2337-250, ATGP2337-500, ATGP2337-1

Gjerstorff MF, et al. (2008) . Tissue Antigens.71 (3) :187-92. ; ; De Backer O, Arden KC, et al. (1999) . Cancer Res. 59 (13) :3157-65.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Cancer

NP_001120684.1

Q6NT46

Human

MSWRGRS TYRPRPRRYV EPPEMIGPMR PEQFSDEVEP ATPEEGEPAT QRQDPAAAQE GQDEGASAGQ GPKPEAHSQE QGHPQTGCEC EDGPDGQEMD PPNPEEVKTP EEGEKQSQC