Recombinant humaan GAGE2D-eiwit

GAGE2D, as known as G antigen 2D, belongs to a family of proteins organized in clustered repeats. They have a high degree of predicted sequence identity, but differ by scattered single nucleotide substitution. The first GAGE nomenclature was based on identified mRNA sequences, but the high identity of the GAGE members made impossible to separate products of paralogous genes from polymorph products. Recombinant human GAGE2D protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

G antigen 2D, CT4.8, GAGE-2D, GAGE-8, GAGE8

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

> 95% by SDS-PAGE

15.2 kDa (139aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

GAGE2D, G antigen 2D, CT4.8, GAGE-2D, GAGE-8, GAGE8, ATGP2364-10 µg, ATGP2364-20 µg, ATGP2364-50 µg, ATGP2364-100 µg, ATGP2364-250 µg, ATGP2364-500 µg, ATGP2364-1 mg, ATGP2364-10, ATGP2364-20, ATGP2364-50, ATGP2364-100, ATGP2364-250, ATGP2364-500, ATGP2364-1

Backer O. et al. (1999) Cancer Res. 59:3157-3165; ; Gjerstorff MF. et al. (2008) Tissue Antigens. 71:187-192.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Cancer

NP_001091877

Q9UEU5

Human

MSWRGRS TYRPRPRRYV EPPEMIGPMR PEQFSDEVEP ATPEEGEPAT QRQDPAAAQE GEDEGASAGQ GPKPEADSQE QGHPQTGCEC EDGPDGQEMD PPNPEEVKTP EEGEKQSQC