Recombinant humaan Galectine-13/LGALS13-eiwit

Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene has lysophospholipase activity. It is composed of two identical subunits which are held together by disulfide bonds. This protein has structural similarity to several members of the beta-galactoside-binding S-type lectin family. Recombinant human LGALS13 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Galactoside-binding soluble lectin 13, GAL13, PLAC8, PP13, Placental tissue protein 13, Placental protein 13

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

18.5 kDa (162aa) confirmed by MALDI-TOF

GAL13, Galactoside-binding soluble lectin 13, LGALS13, PLAC8, Placental protein13, Placental protein 13, Placental tissue protein 13, Placental tissue protein13, PP13, ATGP2493-10 µg, ATGP2493-20 µg, ATGP2493-50 µg, ATGP2493-100 µg, ATGP2493-250 µg, ATGP2493-500 µg, ATGP2493-1 mg, ATGP2493-010, ATGP2493-020, ATGP2493-050, ATGP2493-100, ATGP2493-250, ATGP2493-500, ATGP2493-01M

Than NG, Pick E, et al. (2004) . Eur J Biochem. 271 (6) :1065-78.; ; Visegrady B, Than NG, et al. (2001) . Protein Eng. 14 (11) :875-80.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Metabolism

NP_037400

Q9UHV8

Human

MGSSHHHHHH SSGLVPRGSH MGSMSSLPVP YKLPVSLSVG SCVIIKGTPI HSFINDPQLQ VDFYTDMDED SDIAFRFRVH FGNHVVMNRR EFGIWMLEET TDYVPFEDGK QFELCIYVHY NEYEIKVNGI RIYGFVHRIP PSFVKMVQVS RDISLTSVCV CN