Recombinant humaan Galectine-8/LGALS8-eiwit

LGALS8, also known as prostate-specific membrane antigen (PCTA1), is an additional prostate-specific antigen that is overexpressed only in malignant tumors and therefore is a more specific identifier of malignancies. It is a member of the galectin gene family which mediates both cell-cell and cell matrix interactions in a manner similar to the selectin subgroup of C-type lectins. Recombinant human LGALS8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Measured by its ability to agglutinate human red blood cells. The ED50 range ≤ 10 µg/mL.

Prostate carcinoma tumor antigen 1, Po66-CBP, Po66 carbohydrate-binding protein, PCTA1, Galectin-8, Gal-8

E.coli

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Measured by its ability to agglutinate human red blood cells. The ED50 range ≤ 10 µg/mL.

37.9 kDa (337aa) confirmed by MALDI-TOF

Galectin8, Galectin-8, Gal-8, PCTA1, Po66-CBP, Prostate carcinoma tumor antigen 1, Galectin-8, ATGP0688-10 µg, ATGP0688-20 µg, ATGP0688-50 µg, ATGP0688-100 µg, ATGP0688-250 µg, ATGP0688-500 µg, ATGP0688-1 mg, ATGP0688-10, ATGP0688-20, ATGP0688-50, ATGP0688-100, ATGP0688-250, ATGP0688-500, ATGP0688-1

Kobayashi T., et al. (2010) Leukemia. 24 (4) :843-50.; ; Streetly MJ., et al. (2010) Blood. 115 (19) :3939-48.;

Suzuki O et al., Sialylation by B-galactoside a-2,6-sialyltransferase and N-glycans regulate cell adhesion and invasion in human anaplastic large cell lymphoma. (Int J Oncol. 2015) {https://pubmed.ncbi.nlm.nih.gov/25573487/}; ; Campanero-Rhodes, M.A, et al. Exploration of Galectin Ligands Displayed on Gram-Negative Respiratory Bacterial Pathogens with Different Cell Surface Architectures. (Biomolecules. 2021) {https://pubmed.ncbi.nlm.nih.gov/33919637/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M Nacl, 1mM DTT, 10% glycerol

Cancer

NP_963837

O00214

Human

MMLSLNNLQN IIYNPVIPFV GTIPDQLDPG TLIVIRGHVP SDADRFQVDL QNGSSMKPRA DVAFHFNPRF KRAGCIVCNT LINEKWGREE ITYDTPFKRE KSFEIVIMVL KDKFQVAVNG KHTLLYGHRI GPEKIDTLGI YGKVNIHSIG FSFSSDLQST QASSLELTEI SRENVPKSGT PQLRLPFAAR LNTPMGPGRT VVVKGEVNAN AKSFNVDLLA GKSKDIALHL NPRLNIKAFV RNSFLQESWG EEERNITSFP FSPGMYFEMI IYCDVREFKV AVNGVHSLEY KHRFKELSSI DTLEINGDIH LLEVRSW