Recombinant humaan GALK1-eiwit

GALK1, also often designated galactokinase, is important in the first step of the galactose metabolism pathway. (ATP + D-galactose = ADP + alpha-D-galactose 1-phosphate) Defects in GALK1 are the cause of galactosemia II. It is an autosomal recessive deficiency characterized by congenital cataracts during infancy and presenile cataracts in the adult population. The cataracts are secondary to accumulation of galactitol in the lenses. Recombinant human GALK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Galactokinase, GALK, GK1, Galactokinase

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

44.4 (412aa) confirmed by MALDI-TOF

GALK1, Galactokinase, GALK, GK1, Galactokinase, ATGP0641-10 µg, ATGP0641-20 µg, ATGP0641-50 µg, ATGP0641-100 µg, ATGP0641-250 µg, ATGP0641-500 µg, ATGP0641-1 mg, ATGP0641-10, ATGP0641-20, ATGP0641-50, ATGP0641-100, ATGP0641-250, ATGP0641-500, ATGP0641-1

Ai Y., et al. (1995) Biochem Biophys Res Commun. 212 (2) :687-91.; ; Hunter M., et al. (2001) Hum Mutat. 17 (1) :77-8.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT

Enzymes & Proteases

NP_000145.1

P51570

Human

MAALRQPQVA ELLAEARRAF REEFGAEPEL AVSAPGRVNL IGEHTDYNQG LVLPMALELM TVLVGSPRKD GLVSLLTTSE GADEPQRLQF PLPTAQRSLE PGTPRWANYV KGVIQYYPAA PLPGFSAVVV SSVPLGGGLS SSASLEVATY TFLQQLCPDS GTIAARAQVC QQAEHSFAGM PCGIMDQFIS LMGQKGHALL IDCRSLETSL VPLSDPKLAV LITNSNVRHS LASSEYPVRR RQCEEVARAL GKESLREVQL EELEAARDLV SKEGFRRARH VVGEIRRTAQ AAAALRRGDY RAFGRLMVES HRSLRDDYEV SCPELDQLVE AALAVPGVYG SRMTGGGFGG CTVTLLEASA APHAMRHIQE HYGGTATFYL SQAADGAKVL CL