Recombinant humaan GALM-eiwit

GALM (Galactose mutarotase), also known as Aldose 1-epimerase, is a key enzyme of carbohydrate metabolism catalysing the conversion of beta-D-galactose to alpha-D-galactose. This protein may be essential for normal galactose metabolism by maintaining the equilibrium of alpha and beta anomers of galactose. Also it is required for the production of complex oligosaccharides. Recombinant human GALM, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Galctose mutarotase, Aldose 1-epimerase, BLOCK25, IBD1, Galctose mutarotase, Aldose1-epimerase, BLOCK 25, BLOCK-25

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

39.9 kDa (362aa) confirmed by MALDI-TOF

GALM, Galctose mutarotase, Aldose 1-epimerase, BLOCK25, IBD1, Galctose mutarotase, Aldose1-epimerase, BLOCK 25, BLOCK-25, ATGP0620-10 µg, ATGP0620-20 µg, ATGP0620-50 µg, ATGP0620-100 µg, ATGP0620-250 µg, ATGP0620-500 µg, ATGP0620-1 mg, ATGP0620-10, ATGP0620-20, ATGP0620-50, ATGP0620-100, ATGP0620-250, ATGP0620-500, ATGP0620-1

Ross AC., et al. (2007) Biochemistry. 46 (51) :15198-207; ; Reece RJ., et al (2003) FEBS Lett. 543 (1-3) :21-4.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Metabolism

NP_620156

Q96C23

Human

MASVTRAVFG ELPSGGGTVE KFQLQSDLLR VDIISWGCTI TALEVKDRQG RASDVVLGFA ELEGYLQKQP YFGAVIGRVA NRIAKGTFKV DGKEYHLAIN KEPNSLHGGV RGFDKVLWTP RVLSNGVQFS RISPDGEEGY PGELKVWVTY TLDGGELIVN YRAQASQATP VNLTNHSYFN LAGQASPNIN DHEVTIEADT YLPVDETLIP TGEVAPVQGT AFDLRKPVEL GKHLQDFHLN GFDHNFCLKG SKEKHFCARV HHAASGRVLE VYTTQPGVQF YTGNFLDGTL KGKNGAVYPK HSGFCLETQN WPDAVNQPRF PPVLLRPGEE YDHTTWFKFS VA