Recombinant humaan GalNac transferase 1/GALNT1-eiwit

GALNT1, also known as polypeptide N-acetylgalactosaminyltransferase 1, is a member of the GalNAc-T family of enzymes. It utilizes UDP-GalNAc as the nucleotide donor substrate to modify serine or threonine residues of proteins trafficking through the secretory pathway. Structurally, GalNAc-Ts consist of an N-terminal catalytic domain tethered by a short linker to a C-terminal ricin-like lectin domain containing three potential carbohydrate-binding sites. Also, this protein is involved in the glycosylation of proteins essential for bone formation such as osteopontin and bone sialoprotein. Recombinant human GALNT1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that transfer 1.0 pmole of GalNAc from UDP-GalNAc to peptide EA2 per minute per minute at pH 8.0 at 37C.

Polypeptide N-acetylgalactosaminyltransferase 1, Polypeptide GalNAc transferase 1, GalNAc-T1, pp-GaNTase 1, Protein-UDP acetylgalactosaminyltransferase 1, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1

Baculovirus

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 300pmol/min/ug, and is defined as the amount of enzyme that transfer 1.0pmole of GalNAc from UDP-GalNAc to peptide EA2 per minute per minute at pH 8.0 at 37C.

60.4 kDa (528aa)

GALNT1, GALNT1Polypeptide N-acetylgalactosaminyltransferase 1, GALNT1, GALNAC-T1, ATGP3850-10 µg, ATGP3850-20 µg, ATGP3850-50 µg, ATGP3850-100 µg, ATGP3850-250 µg, ATGP3850-500 µg, ATGP3850-1 mg, ATGP3850-10, ATGP3850-20, ATGP3850-50, ATGP3850-100, ATGP3850-250, ATGP3850-500, ATGP3850-1

Gerken, T.A. et al. (2011) J. Biol. Chem. 286:14493-14507.; ; Partt, M.R. et al. (2004) Chem. Biol. 11:1009-1016.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Signal Transduction

NP_065207

Q10472

Human

Ala

GLPAGDV LEPVQKPHEG PGEMGKPVVI PKEDQEKMKE MFKINQFNLM ASEMIALNRS LPDVRLEGCK TKVYPDNLPT TSVVIVFHNE AWSTLLRTVH SVINRSPRHM IEEIVLVDDA SERDFLKRPL ESYVKKLKVP VHVIRMEQRS GLIRARLKGA AVSKGQVITF LDAHCECTVG WLEPLLARIK HDRRTVVCPI IDVISDDTFE YMAGSDMTYG GFNWKLNFRW YPVPQREMDR RKGDRTLPVR TPTMAGGLFS IDRDYFQEIG TYDAGMDIWG GENLEISFRI WQCGGTLEIV TCSHVGHVFR KATPYTFPGG TGQIINKNNR RLAEVWMDEF KNFFYIISPG VTKVDYGDIS SRVGLRHKLQ CKPFSWYLEN IYPDSQIPRH YFSLGEIRNV ETNQCLDNMA RKENEKVGIF NCHGMGGNQV FSYTANKEIR TDDLCLDVSK LNGPVTMLKC HHLKGNQLWE YDPVKLTLQH VNSNQCLDKA TEEDSQVPSI RDCNGSRSQQ WLLRNVTLPE IF