Recombinant humaan GALT-eiwit

GALT catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of uDP-glucose + galactose-1-phosphate to glucose-1-phosphate + uDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human GALT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Galactose-1-phosphate uridylyltransferase, Gal-1-P uridylyltransferase, uDP-glucose--hexose-1-phosphate uridylyltransferase

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

45.9 kDa (403aa) confirmed by MALDI-TOF

GALT, Galactose-1-phosphate uridylyltransferase, Gal-1-P uridylyltransferase, uDP-glucose--hexose-1-phosphate uridylyltransferase, ATGP1904-10 µg, ATGP1904-20 µg, ATGP1904-50 µg, ATGP1904-100 µg, ATGP1904-250 µg, ATGP1904-500 µg, ATGP1904-1 mg, ATGP1904-10, ATGP1904-20, ATGP1904-50, ATGP1904-100, ATGP1904-250, ATGP1904-500, ATGP1904-1

Reichardt JK. (1992) Hum Mutat. (3) :190-6.; ; Tyfield L, Reichardt J, et al. (1999) . Hum Mutat. (6) :417-30.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 10% glycerol

Metabolism

NP_000146.2

P07902

Human

MSRSGT DPQQRQQASE ADAAAATFRA NDHQHIRYNP LQDEWVLVSA HRMKRPWQGQ VEPQLLKTVP RHDPLNPLCP GAIRANGEVN PQYDSTFLFD NDFPALQPDA PSPGPSDHPL FQAKSARGVC KVMCFHPWSD VTLPLMSVPE IRAVVDAWAS VTEELGAQYP WVQIFENKGA MMGCSNPHPH CQVWASSFLP DIAQREERSQ QAYKSQHGEP LLMEYSRQEL LRKERLVLTS EHWLVLVPFW ATWPYQTLLL PRRHVRRLPE LTPAERDDLA SIMKKLLTKY DNLFETSFPY SMGWHGAPTG SEAGANWNHW QLHAHYYPPL LRSATVRKFM VGYEMLAQAQ RDLTPEQAAE RLRALPEVHY HLGQKDRETA TIA