Recombinant humaan Gamma-glutamylcyclotransferase/GGCT-eiwit

GGCT, also known as gamma-glutamylcyclotransferase, belongs to the family of transferases, specifically the aminoacyltransferases. This protein catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides and may play a significant role in glutathione homeostasis. GGCT induces release of cytochrome c from mitochondria with resultant induction of apoptosis. Recombinant human GGCT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Gamma-glutamylcyclotransferase, C7orf24, CRF21, FLJ11717, GCTG, Ggc, MGC3077, Cytochrome c-releasing factor 21

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

> 95% by SDS-PAGE

23.2 kDa (208aa) confirmed by MALDI-TOF

G-Glutamylcyclotransferase, CRF21, Gamma-glutamylcyclotransferase, C7orf24, CRF21, FLJ11717, GCTG, Ggc, MGC3077, ATGP0887-10 µg, ATGP0887-20 µg, ATGP0887-50 µg, ATGP0887-100 µg, ATGP0887-250 µg, ATGP0887-500 µg, ATGP0887-1 mg, ATGP0887-10, ATGP0887-20, ATGP0887-50, ATGP0887-100, ATGP0887-250, ATGP0887-500, ATGP0887-1

Masuda Y., et al. (2006) Biochem Biophys Res Commun. 346 (2) :454-60. ; ; Oakley AJ., et al. (2008) J Biol Chem. 283 (32) :22031-42.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Enzymes & Proteases

NP_076956

O75223

Human

MGSSHHHHHH SSGLVPRGSH MANSGCKDVT GPDEESFLYF AYGSNLLTER IHLRNPSAAF FCVARLQDFK LDFGNSQGKT SQTWHGGIAT IFQSPGDEVW GVVWKMNKSN LNSLDEQEGV KSGMYVVIEV KVATQEGKEI TCRSYLMTNY ESAPPSPQYK KIICMGAKEN GLPLEYQEKL KAIEPNDYTG KVSEEIEDII KKGETQTL