Recombinant humaan GAMT-eiwit

Guanidinoacetate N-methyltransferase isoform a, also known as GAMT is a methyltansferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl domor. This enzyme participates in the two-step synthesis of the compound creatine from the protein building blocks glycine, arginine, and methionine. It is involved in providing energy for muscle contraction, and is also important in nervous system functioning. Also GAMT is active in the liver, pancreas, and kidneys. Recombinant human GAMT, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Guanidinoacetate N-methyltransferase isoform a, PIG2, TP53I2, Guanidinoacetate N-methyltransferase isoform a Guanidinoacetate N methyltransferase.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

28.4 kDa (256aa) confirmed by MALDI-TOF

GAMT, Guanidinoacetate N-methyltransferase isoform a, PIG2, TP53I2, Guanidinoacetate N-methyltransferase isoform a Guanidinoacetate N methyltransferase., ATGP0424-10 µg, ATGP0424-20 µg, ATGP0424-50 µg, ATGP0424-100 µg, ATGP0424-250 µg, ATGP0424-500 µg, ATGP0424-1 mg, ATGP0424-10, ATGP0424-20, ATGP0424-50, ATGP0424-100, ATGP0424-250, ATGP0424-500, ATGP0424-1

Almeida LS., et al. (2007) Mol Genet Metab. 91 (1) :1-6. ; ; Jenne DE., et al. (1997) Biochem Biophys Res Commun. 238 (3) :723-7. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,10% glycerol

Enzymes & Proteases

NP_000147

Q14353

Human

MGSSHHHHHH SSGLVPRGSH MSAPSATPIF APGENCSPAW GAAPAAYDAA DTHLRILGKP VMERWETPYM HALAAAASSK GGRVLEVGFG MAIAASKVQE APIDEHWIIE CNDGVFQRLR DWAPRQTHKV IPLKGLWEDV APTLPDGHFD GILYDTYPLS EETWHTHQFN FIKNHAFRLL KPGGVLTYCN LTSWGELMKS KYSDITIMFE ETQVPALLEA GFRRENIRTE VMALVPPADC RYYAFPQMIT PLVTKG