Recombinant humaan GAPDH-eiwit

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36-kDa subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1, 3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recombinant GAPDH protein was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 50 units/mg, and is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.

Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, G3PD

E.coli

Human

Non-Tagged

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 50unit/mg, and is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.

36 kDa (335aa) confirmed by MALDI-TOF

Wu:fb33a10, Peptidyl-cysteine S-nitrosylase GAPDH, Peptidyl cysteine S nitrosylase GAPDH, p38 component, OTTHuMP00000174432, OTTHuMP00000174431, OCT1 coactivator in S phase, OCAS, MGC88685, MGC127711, MGC105239, MGC103191, MGC103190, MGC102546, MGC102544, KNC-NDS6, KNC NDS6, HEL-S-162eP, HEL S 162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2, Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Glyceraldehyde-3-phosphate dehydrogenase 38 kDa BFA-dependent ADP-ribosylation substrate, Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde3 phosphate dehydrogenase, Glyceraldehyde 3 phosphate dehydrogenase isoform 2, Glyceraldehyde 3 phosphate dehydrogenase isoform 1, Glyceraldehyde 3 phosphate dehydrogenase 38 kDa BFA dependent ADP ribosylation substrate, Glyceraldehyde 3 phosphate dehydrogenase, GAPDH human, GAPDH, GAPD, G3PDH, G3PD, EC1.2.1.12, EC 1.2.1.12, cb609, BARS-38, BARS 38, Aging-associated gene 9 protein, Aging associated gene 9 protein, 38-KD component, 38 KD compone

Ralser M., et al. (2007) .J Biol. 6 (4) :10; ; Tisdale Ej., et al. (2007) .Traffic. 8 (6) :733-41

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 1mM EDTA, 20% glycerol

Enzymes & Proteases

NP_002037

P04406

Human

MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE