Recombinant humaan GARS-eiwit

GARS, also known as glycyl-tRNA synthetase, is one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. This enzyme is an (alpha) 2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Recombinant human GARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glycyl-tRNA synthetase, CMT2D, DSMAV, GlyRS, HMN5, SMAD1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

30 kDa (270aa) confirmed by MALDI-TOF

GARS, Glycyl-tRNA synthetase, CMT2D, DSMAV, GlyRS, HMN5, SMAD1, ATGP2756-10 µg, ATGP2756-20 µg, ATGP2756-50 µg, ATGP2756-100 µg, ATGP2756-250 µg, ATGP2756-500 µg, ATGP2756-1 mg, ATGP2756-10, ATGP2756-20, ATGP2756-50, ATGP2756-100, ATGP2756-250, ATGP2756-500, ATGP2756-1

Guo R.-T., et al. (2009) J. Biol. Chem. 284:28968-28976; ; Xie W., et al. (2007) Proc. Natl. Acad. Sci. u.S.A. 104:9976-9981;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_002038

P41250

Human

PISLPAA ASRSSMDGAG AEEVLAPLRL AVRQQGDLVR KLKEDKAPQV DVDKAVAELK ARKRVLEAKE LALQPKDDIV DRAKMEDTLK RRFFYDQAFA IYGGVSGLYD FGPVGCALKN NIIQTWRQHF IQEEQILEID CTMLTPEPVL KTSGHVDKFA DFMVKDVKNG ECFRADHLLK AHLQKLMSDK KCSVEKKSEM ESVLAQLDNY GQQELADLFV NYNVKSPITG NDLSPPVSFN LMFKTFIGPG