Recombinant humaan GATC-eiwit

GATC allows the formation of correctly charged Gln-tRNA (Gln) through the transamidation of misacylated Glu-tRNA (Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA (Gln) . This protein is subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112) and C (GATC) subunits. Recombinant human GATC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glutamyl-tRNA (Gln) amidotransferase subunit C homolog, Glutamyl-tRNA (Gln) amidotransferase, subunit C homolog, 15E1.2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

17.5 kDa (159aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

GATC, Glutamyl-tRNA (Gln) amidotransferase subunit C homolog, Glutamyl-tRNA (Gln) amidotransferase, subunit C homolog, 15E1.2, ATGP2183-10 µg, ATGP2183-20 µg, ATGP2183-50 µg, ATGP2183-100 µg, ATGP2183-250 µg, ATGP2183-500 µg, ATGP2183-1 mg, ATGP2183-10, ATGP2183-20, ATGP2183-50, ATGP2183-100, ATGP2183-250, ATGP2183-500, ATGP2183-1

Nagao A., et al. (2009) Proc. Natl. Acad. Sci. u.S.A. 106:16209-16214

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Enzymes & Proteases

NP_789788

O43716

Human

MWSRLVW LGLRAPLGGR QGFTSKADPQ GSGRITAAVI EHLERLALVD FGSREAVARL EKAIAFADRL RAVDTDGVEP MESVLEDRCL YLRSDNVVEG NCADELLQNS HRVVEEYFVA PPGNISLPKL DEQEPFPHS