Recombinant humaan GATE-16 eiwit

GABARAPL2, also known as ATG8, is involved in intra Golgi traffic and modulates intra Golgi transport through coupling between NSF activity and SNAREs activation. GABARAPL2 first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. Recombinant human GABARAPL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Gamma-aminobutyric acid receptor-associated protein-like 2, GABA (A) receptor-associated protein-like 2, ATG8, ATG8C, GATE-16, GATE16, GEF2, FLC3A

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

15.8 kDa (137aa) confirmed by MALDI-TOF

GEF-2, GEF2, GEF 2, GATE-16, GATE16, GATE 16, Gamma-aminobutyric acid receptor-associated protein-like 2, Gamma aminobutyric acid receptor associated protein like 2, GABARAPL2, GABA (A) receptor-associated protein-like 2, GABA (A) receptor associated protein like 2, GABA type A receptor associated protein like 2, FLC3A, ATG8C, ATG8, ATGP0885-10 µg, ATGP0885-20 µg, ATGP0885-50 µg, ATGP0885-100 µg, ATGP0885-250 µg, ATGP0885-500 µg, ATGP0885-1 mg, ATGP0885-010, ATGP0885-020, ATGP0885-050, ATGP0885-100, ATGP0885-250, ATGP0885-500, ATGP0885-01M

Behrends C., et al. (2010) Nature. 466 (7302) :68-76.; ; Sengupta A., et al. (2009) J Biol Chem. 284 (41) :28319-31.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol

Cancer

NP_009216

P60520

Human

MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL VPSDITVAQF MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD EDGFLYVAYS GENTFGF