Recombinant humaan GATM-eiwit

GATM (Glycine amidinotransferase, mitochondrial) is a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Creatine plays a vital role in energy metabolism in muscle tissues. It plays a role in embryonic and central nervous system development. Recombinant human GATM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glycine amidinotransferase mitochondrial, glycine amidinotransferase, mitochondrial, AGAT, AT

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

46.9 kDa (410aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

GATM, glycine amidinotransferase mitochondrial, glycine amidinotransferase, mitochondrial, AGAT, AT, ATGP1438-10 µg, ATGP1438-20 µg, ATGP1438-50 µg, ATGP1438-100 µg, ATGP1438-250 µg, ATGP1438-500 µg, ATGP1438-1 mg, ATGP1438-10, ATGP1438-20, ATGP1438-50, ATGP1438-100, ATGP1438-250, ATGP1438-500, ATGP1438-1

Schulze A (2003) . Mol. Cell. Biochem. 244 (1-2) : 143-50.; ; Cullen M.E., Et al. (2006) Circulation 114:I16-I20;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200 mM NaCl

Metabolism

NP_001473.1

P50440

Human

STQAAT ASSRNSCAAD DKATEPLPKD CPVSSYNEWD PLEEVIVGRA ENACVPPFTI EVKANTYEKY WPFYQKQGGH YFPKDHLKKA VAEIEEMCNI LKTEGVTVRR PDPIDWSLKY KTPDFESTGL YSAMPRDILI VVGNEIIEAP MAWRSRFFEY RAYRSIIKDY FHRGAKWTTA PKPTMADELY NQDYPIHSVE DRHKLAAQGK FVTTEFEPCF DAADFIRAGR DIFAQRSQVT NYLGIEWMRR HLAPDYRVHI ISFKDPNPMH IDATFNIIGP GIVLSNPDRP CHQIDLFKKA GWTIITPPTP IIPDDHPLWM SSKWLSMNVL MLDEKRVMVD ANEVPIQKMF EKLGITTIKV NIRNANSLGG GFHCWTCDVR RRGTLQSYLD