Recombinant humaan GCA-eiwit

GCA, also known as Grancalcin, is calcium-binding protein that is especially abundant in human neutrophils. It belongs to the penta EF-hand (PEF) subfamily of EF-hand proteins, which also comprises calpain, sorcin, peflin, and ALG-2. This protein undergoes important conformational changes upon binding of calcium, which subsequently exposes hydrophobic amino acid residues, which direct the protein to hydrophobic surfaces. It interacts with L-plastin, a protein known to have actin bundling activity, which suggests that GCA may play a role in the regulation of neutrophils adhesion. Recombinant human GCA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Grancalcin, GCL

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

> 95% by SDS-PAGE

26.1 kDa (237aa) confirmed by MALDI-TOF

GCA, Grancalcin, GCL, ATGP0886-10 µg, ATGP0886-20 µg, ATGP0886-50 µg, ATGP0886-100 µg, ATGP0886-250 µg, ATGP0886-500 µg, ATGP0886-1 mg, ATGP0886-10, ATGP0886-20, ATGP0886-50, ATGP0886-100, ATGP0886-250, ATGP0886-500, ATGP0886-1

Teahan C.G., et al. (1992) . Biochem. J., 286: 549-554.; ; Boyhan A., et al. (1992) . J. Biol. Chem., 267: 2928-2933.

Jung SH, et al. Integrative proteomic profiling of protein activity and interactions using protein arrays. (Mol Cell Proteomics. 2012) {https://pubmed.ncbi.nlm.nih.gov/22843993/}; ; Jung SH, et al. Identification of transglutaminase 2 kinase substrates using a novel on-chip activity assay. (Biosens Bioelectron. 2016) {https://pubmed.ncbi.nlm.nih.gov/27040940/}; ; Jung SH, et al. Systematic investigation of protein kinase A substrate proteins using on-chip protein kinase kinetic profiling. (Analyst. 2017) {https://pubmed.ncbi.nlm.nih.gov/28536714/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl

Signal Transduction

NP_036330.1

P28676

Human

MAYPGYGGGF GNFSIQVPGM QMGQPVPETG PAILLDGYSG PAYSDTYSSA GDSVYTYFSA VAGQDGEVDA EELQRCLTQS GINGTYSPFS LETCRIMIAM LDRDHTGKMG FNAFKELWAA LNAWKENFMT VDQDGSGTVE HHELRQAIGL MGYRLSPQTL TTIVKRYSKN GRIFFDDYVA CCVKLRALTD FFRKRDHLQQ GSANFIYDDF LQGTMAI