Recombinant humaan GCHFR-eiwit

GTP cyclohydrolase I feedback regulatory protein (GCHFR) binds to and mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase I. The regulatory protein, GCHFR, consists of a homodimer. It is postulated that GCHFR may play a role in regulating phenylalanine metabolism in the liver and in the production of biogenic amine neurotransmitters and nitric oxide. Recombinant human GCHFR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

GTP cyclohydrolase I feedback regulatory protein, GFRP, HsT16933, P35

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

12.1 kDa (107aa) confirmed by MALDI-TOF

GCHFR, GTP cyclohydrolase I feedback regulatory protein, GFRP, HsT16933, P35, ATGP2738-10 µg, ATGP2738-20 µg, ATGP2738-50 µg, ATGP2738-100 µg, ATGP2738-250 µg, ATGP2738-500 µg, ATGP2738-1 mg, ATGP2738-10, ATGP2738-20, ATGP2738-50, ATGP2738-100, ATGP2738-250, ATGP2738-500, ATGP2738-1

Chavan B., et al. (2006) J. Invest. Dermatol. 126:2481-2489; ; Swick L., et al. (2006) J. Neurochem. 97:1447-1455

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 40% glycerol, 1mM DTT

Metabolism

NP_005249

P30047

Human

MGSSHHHHHH SSGLVPRGSH MGSMPYLLIS TQIRMEVGPT MVGDEQSDPE LMQHLGASKR RALGNNFYEY YVDDPPRIVL DKLERRGFRV LSMTGVGQTL VWCLHKE