Recombinant humaan GCHH-eiwit

Glutaryl-CoA dehydrogenase (GCDH) belongs to the acyl-CoA dehydrogenase family. GCDH catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO (2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. This Protein uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. Recombinant GCDH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glutaryl-CoA dehydrogenase, ACAD5, GCD, Glutaryl-CoA dehydrogenase, ACAD-5, ACAD 5

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

45.7 kDa (415aa) confirmed by MALDI-TOF

GCDH, Glutaryl-CoA dehydrogenase, ACAD5, GCD, Glutaryl-CoA dehydrogenase, ACAD-5, ACAD 5, ATGP0622-10 µg, ATGP0622-20 µg, ATGP0622-50 µg, ATGP0622-100 µg, ATGP0622-250 µg, ATGP0622-500 µg, ATGP0622-1 mg, ATGP0622-10, ATGP0622-20, ATGP0622-50, ATGP0622-100, ATGP0622-250, ATGP0622-500, ATGP0622-1

Keyser B., et al. (2008) Hum. Mol. Genet. 17:3854-3863; ; Westover JB., et al. (2001) Biochemistry. 40 (46) :14106-14.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 200 mM NaCl

Metabolism

NP_000150

Q92947

Human

MRPEFDWQDP LVLEEQLTTD EILIRDTFRT YCQERLMPRI LLANRNEVFH REIISEMGEL GVLGPTIKGY GCAGVSSVAY GLLARELERV DSGYRSAMSV QSSLVMHPIY AYGSEEQRQK YLPQLAKGEL LGCFGLTEPN SGSDPSSMET RAHYNSSNKS YTLNGTKTWI TNSPMADLFV VWARCEDGCI RGFLLEKGMR GLSAPRIQGK FSLRASATGM IIMDGVEVPE ENVLPGASSL GGPFGCLNNA RYGIAWGVLG ASEFCLHTAR QYALDRMQFG VPLARNQLIQ KKLADMLTEI TLGLHACLQL GRLKDQDKAA PEMVSLLKRN NCGKALDIAR QARDMLGGNG ISDEYHVIRH AMNLEAVNTY EGTHDIHALI LGRAITGIQA FTASK