Recombinant humaan GCLC-eiwit (Trx-tag)

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

GCLC

Glutamate-cysteine ligase catalytic subunit; Gamma-ECS; GCS heavy chain

P48506

P48506

E.coli

N-Trx

Met1-Val252

Metabolism; Cell Biology; Cancer

< 10 EU/mg of the protein as determined by the LAL method

> 95% as determined by reducing SDS-PAGE.

Not validated for activity

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

This product is provided as lyophilized powder which is shipped with ice packs.

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

https://789.bio/eb/ae5C4G

47.6 kDa

47 kDa

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Human