Recombinant humaan GCLM-eiwit

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Recombinant human GCLM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glutamate-cysteine ligase modifier subunit, Glutamate-cysteine ligase, modifier subunit, GLCLR

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

33.3 kDa (298aa) confirmed by MALDI-TOF

GCLM, Glutamate-cysteine ligase modifier subunit, Glutamate-cysteine ligase, modifier subunit, GLCLR, ATGP1657-10 µg, ATGP1657-20 µg, ATGP1657-50 µg, ATGP1657-100 µg, ATGP1657-250 µg, ATGP1657-500 µg, ATGP1657-1 mg, ATGP1657-10, ATGP1657-20, ATGP1657-50, ATGP1657-100, ATGP1657-250, ATGP1657-500, ATGP1657-1

Hanzawa, R., et al. (2011) Psychiatry Clin. Neurosci. 65 (1), 39-46

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl

Metabolism

NP_002052

P48507

Human

MGTDSR AAKALLARAR TLHLQTGNLL NWGRLRKKCP STHSEELHDC IQKTLNEWSS QINPDLVREF PDVLECTVSH AVEKINPDER EEMKVSAKLF IVESNSSSST RSAVDMACSV LGVAQLDSVI IASPPIEDGV NLSLEHLQPY WEELENLVQS KKIVAIGTSD LDKTQLEQLY QWAQVKPNSN QVNLASCCVM PPDLTAFAKQ FDIQLLTHND PKELLSEASF QEALQESIPD IQAHEWVPLW LLRYSVIVKS RGIIKSKGYI LQAKRRGS