Recombinant humaan GCSH-eiwit

The enzyme system for cleavage of glycine (glycine cleavage system) is composed of four mitochondrial protein components: P protein, H protein, T protein, and L protein. GCSH is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH) . Recombinant human GCSH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glycine cleavage system protein H (aminomethyl carrier), GCE, NKH

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

16.4 kDa (149aa) confirmed by MALDI-TOF

GCSH, Glycine cleavage system protein H (aminomethyl carrier), GCE, NKH, ATGP1281-10 µg, ATGP1281-20 µg, ATGP1281-50 µg, ATGP1281-100 µg, ATGP1281-250 µg, ATGP1281-500 µg, ATGP1281-1 mg, ATGP1281-10, ATGP1281-20, ATGP1281-50, ATGP1281-100, ATGP1281-250, ATGP1281-500, ATGP1281-1

Kure S., et al. (2001) J Hum Genet. 46 (7) :378-84.; ; Kovata H., et al. (1991) Am J Hum Genet. 48 (2) :351-61.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.

Metabolism

NP_004474

P23434

Human

SVRKFT EKHEWVTTEN GIGTVGISNF AQEALGDVVY CSLPEVGTKL NKQDEFGALE SVKAASELYS PLSGEVTEIN EALAENPGLV NKSCYEDGWL IKMTLSNPSE LDELMSEEAY EKYIKSIEE