Recombinant humaan GDI2-eiwit

Rab GDP dissociation inhibitor beta isoform 1, also known as GDI2, belongs to the GDP dissociation inhibitors (GDIs) family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. Recombinant human GDI2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Rab GDP dissociation inhibitor beta isoform 1, Rab GDP dissociation inhibitor beta isoform 1, RABGDIB

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

53.1 kDa (468aa)

GDI2, Rab GDP dissociation inhibitor beta isoform 1, Rab GDP dissociation inhibitor beta isoform 1, RABGDIB, ATGP2654-10 µg, ATGP2654-20 µg, ATGP2654-50 µg, ATGP2654-100 µg, ATGP2654-250 µg, ATGP2654-500 µg, ATGP2654-1 mg, ATGP2654-10, ATGP2654-20, ATGP2654-50, ATGP2654-100, ATGP2654-250, ATGP2654-500, ATGP2654-1

Nishimura N., et al. (1995) Cancer Res. 55: 5445-5450.; ; Sedlacek Z., et al. (1999) Mol Biol Evol. 16: 1231-1237.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Signal Transduction

NP_001485

P50395

Human

MGSSHHHHHH SSGLVPRGSH MGSMNEEYDV IVLGTGLTEC ILSGIMSVNG KKVLHMDRNP YYGGESASIT PLEDLYKRFK IPGSPPESMG RGRDWNVDLI PKFLMANGQL VKMLLYTEVT RYLDFKVTEG SFVYKGGKIY KVPSTEAEAL ASSLMGLFEK RRFRKFLVYV ANFDEKDPRT FEGIDPKKTT MRDVYKKFDL GQDVIDFTGH ALALYRTDDY LDQPCYETIN RIKLYSESLA RYGKSPYLYP LYGLGELPQG FARLSAIYGG TYMLNKPIEE IIVQNGKVIG VKSEGEIARC KQLICDPSYV KDRVEKVGQV IRVICILSHP IKNTNDANSC QIIIPQNQVN RKSDIYVCMI SFAHNVAAQG KYIAIVSTTV ETKEPEKEIR PALELLEPIE QKFVSISDLL VPKDLGTESQ IFISRTYDAT THFETTCDDI KNIYKRMTGS EFDFEEMKRK KNDIYGED