Recombinant humaan GFER/ALR-eiwit

FAD-linked sulfhydryl oxidase ALR, also known as GFER, belongs to the Erv1/ALR family of proteins. This family can be found in higher and lower eukaryotes. GFER is a hepatotrophic growth factor and flavin-linked sulfhydryl oxidase expressed in various tissues. Also, GFER induces the expression of S-adenosylmethionine decarboxyl-ase and ornithine decarboxylases (ODC), which each play an important role in the synthesis of polyamines. Recombinant human GFER protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

FAD-linked sulfhydryl oxidase ALR, ALR, ERV1, HERV1, HPO, HPO1, HPO2, HSS

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26 kDa (229aa) confirmed by MALDI-TOF

GFER, ALR, FAD-linked sulfhydryl oxidase ALR, ALR, ERV1, HERV1, HPO, HPO1, HPO2, HSS, ATGP1862-10 µg, ATGP1862-20 µg, ATGP1862-50 µg, ATGP1862-100 µg, ATGP1862-250 µg, ATGP1862-500 µg, ATGP1862-1 mg, ATGP1862-10, ATGP1862-20, ATGP1862-50, ATGP1862-100, ATGP1862-250, ATGP1862-500, ATGP1862-1

Thaler W E., et al. (2005) Histopathology. 47:57-66; ; Farrell S R., et al. (2005) Biochemistry. 44: 1532-1541.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT

Signal Transduction

NP_005253

P55789

Human

MAAPGE RGRFHGGNLF FLPGGARSEM MDDLATDARG RGAGRRDAAA SASTPAQAPT SDSPVAEDAS RRRPCRACVD FKTWMRTQQK RDTKFREDCP PDREELGRHS WAVLHTLAAY YPDLPTPEQQ QDMAQFIHLF SKFYPCEECA EDLRKRLCRN HPDTRTRACF TQWLCHLHNE VNRKLGKPDF DCSKVDERWR DGWKDGSCD