Recombinant humaan GFPT1-eiwit

GFPT1 is the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. Recombinant human GFPT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glutamine--fructose-6-phosphate transaminase 1, CMSTA1, GFA, GFAT, GFAT 1, GFAT1, GFAT1m, GFPT, GFPT1L, MSLG

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BRADFORD assay)

> 95% by SDS-PAGE

43.7 kDa (391aa) confirmed by MALDI-TOF

GFPT1, Glutamine--fructose-6-phosphate transaminase 1, CMSTA1, GFA, GFAT, GFAT 1, GFAT1, GFAT1m, GFPT, GFPT1L, MSLG, ATGP2777-10 µg, ATGP2777-20 µg, ATGP2777-50 µg, ATGP2777-100 µg, ATGP2777-250 µg, ATGP2777-500 µg, ATGP2777-1 mg, ATGP2777-10, ATGP2777-20, ATGP2777-50, ATGP2777-100, ATGP2777-250, ATGP2777-500, ATGP2777-1

Selcen, D., et al. (2013) Neurology 81 (4), 370-378 ; ; Huh, S.Y., et al. (2012) Muscle Nerve 46 (4), 600-604

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol, 2mM DTT, 2mM EDTA

Metabolism

NP_001231639

Q06210

Human

QQIMKGN FSSFMQKEIF EQPESVVNTM RGRVNFDDYT VNLGGLKDHI KEIQRCRRLI LIACGTSYHA GVATRQVLEE LTELPVMVEL ASDFLDRNTP VFRDDVCFFL SQSGETADTL MGLRYCKERG ALTVGITNTV GSSISRETDC GVHINAGPEI GVASTKAYTS QFVSLVMFAL MMCDDRISMQ ERRKEIMLGL KRLPDLIKEV LSMDDEIQKL ATELYHQKSV LIMGRGYHYA TCLEGALKIK EITYMHSEGI LAGELKHGPL ALVDKLMPVI MIIMRDHTYA KCQNALQQVV ARQGRPVVIC DKEDTETIKN TKRTIKVPHS VDCLQGILSV IPLQLLAFHL AVLRGYDVDF PRNLAKSVTV E